TnA-directed Deletion of the trp Operon from RSF2124-trp in Escherichia coli

Regulation of trp operon in E . coli

Replacement of the Escherichia coli trp operon attenuation control codons alters operon expression.

To test features of the current model of transcription attenuation in amino acid biosynthetic operons, alterations were introduced into the trp operon leader region and expression of the mutated operons was examined in miaA and miaA+ Escherichia coli strains that lacked the trp repressor. The miaA mutation prevents modification of the adenosine residue immediately 3' of the anticodon of tRNAs t...

Interaction of the trp repressor from Escherichia coli with a constitutive trp operator.

The interaction of the trp repressor from Escherichia coli with a 20 bp fragment of DNA (CGTACTGATT.AATCAGTACG) corresponding to a mutant trp operator was studied by c.d. in the presence and absence of the co-repressor, L-tryptophan, and as a function of the concentration of K+ and Na+ ions. The affinity of the repressor for the mutant operator in the presence of tryptophan is about three order...

Polarity in segments of the Escherichia coli trp operon with deleted intraoperonic translational initiation signals.

The effect of deletion of the operator-distal genes of the trp operon, including the trpE-trpD intercistronic punctuation point, on the degree of transcriptional polarity (in this case the effect of a nonsense mutation on the level of mRNA from the distal part of the very gene where the mutation is located) was investigated. Double mutants which contain a nonsense mutation and a deletion in trp...

Regulation of Trp Operon in E. Coli List of Publications List of Publications from Phd Research

Pragnę złożyć gorące podziękowania, mojemu promotorowi, Panu Profesorowi Robertowi Hołystowi, za cenne uwagi, długie dyskusje i cier-pliwość, dzięki którym powstała niniejsza praca. Rodzicom i siostrze dziękuję za wsparcie i okazane serce. Dedykuję tę pracę mojej żonie. Scaling form of viscosity at all length-scales in poly(ethylene glycol) solutions studied by fluores-cence correlation spectro...